{"type":"video","version":"1.0","html":"<iframe src=\"https://www.loom.com/embed/2aa5a6ddd3374b6d896da54d498d3f3e\" frameborder=\"0\" width=\"1920\" height=\"1440\" webkitallowfullscreen mozallowfullscreen allowfullscreen></iframe>","height":1440,"width":1920,"provider_name":"Loom","provider_url":"https://www.loom.com","thumbnail_height":1440,"thumbnail_width":1920,"thumbnail_url":"https://cdn.loom.com/sessions/thumbnails/2aa5a6ddd3374b6d896da54d498d3f3e-a95124eba3f8aa2e.gif","duration":544.235,"title":"First Genetic Tattoo - Marwan Refaat","description":"The procedure begins with the application of a genetic tattoo using a 6.7 pH solution. The tattoo involves strontium phosphate nanoparticles containing plasmid DNA. Initial efforts focus on finding the appropriate needle angle and depth to avoid procedural errors.\n\nThe experiment progresses with Cherry Red ink applied at a 7.0 pH. Pipetting is performed to handle the ink, with observations noting variations in pellet formation depending on pH levels. The procedure tests four different pH levels for Cherry Red, followed by similar tests for two types of Green ink, each tested at four pH levels.\n\nDuring the process, adjustments are made to optimize the technique, including addressing needle size and angle variations. Incremental increases in pH by 0.3 are applied for each test, with the samples labeled according to the pH level used during preparation. The objective is to study nanoparticle size and aggregation properties across varying pH levels to determine factors influencing efficiency.\n\nThis genetic tattoo employs fluorescent nanoparticles as a biosensor to detect estrogen levels. The nanoparticles are designed to emit a red fluorescence in response to increased estrogen, providing a visual indicator of hormone fluctuations. The process is part of a broader investigation into the efficiency and functionality of biosensing tattoo applications."}