video1.0<iframe src="https://www.loom.com/embed/3c7060ddd42e4c15bab80646f6f03909" frameborder="0" width="1728" height="1296" webkitallowfullscreen mozallowfullscreen allowfullscreen></iframe>12961728Loomhttps://www.loom.com12961728https://cdn.loom.com/sessions/thumbnails/3c7060ddd42e4c15bab80646f6f03909-6b7bda569eabbc76.gif355.035In this tutorial, I walk you through creating custom data transformations in the Data Preparation app using an example with drug data at 0 and 10, including three replicates each. I demonstrate how to upload additional data, add a background subtraction column, and perform column-based arithmetic, including logging and calculating means for each drug condition. We also calculate a signal window by subtracting drug ten from drug zero. I encourage you to follow along and apply these transformations to your own data. Thank you for your attention!