video1.0<iframe src="https://www.loom.com/embed/537bd04a4fa548d4a150ac716f339d55" frameborder="0" width="1728" height="1296" webkitallowfullscreen mozallowfullscreen allowfullscreen></iframe>12961728Loomhttps://www.loom.com12961728https://cdn.loom.com/sessions/thumbnails/537bd04a4fa548d4a150ac716f339d55-84867c07ea4e34a2.gif115.228This Loom explains how to specify backbone modifications in a sequence using industry-standard punctuation. It shows an example where a sulfurizer is called out in the sequence using an asterisk, and notes you can use other punctuation rather than limiting yourself to the asterisk. It emphasizes that you save the sequence, then enter the modification text in the sequence file at the position where the backbone change should occur. The machine may apply changes in reverse order, but placement in the sequence indicates where the modification actually happens.