video1.0<iframe src="https://www.loom.com/embed/5d5dd64692534b678cc31060ecc6c34c" frameborder="0" width="1728" height="1296" webkitallowfullscreen mozallowfullscreen allowfullscreen></iframe>12961728Loomhttps://www.loom.com12961728https://cdn.loom.com/sessions/thumbnails/5d5dd64692534b678cc31060ecc6c34c-00a1140ad5da1ce8.gif549.526This Loom explains the Run Synthesis screen, focusing on how Standard, Meta, and Map protocols control synthesis behavior per run and per column. It details Setup options such as starting at a chosen base, pausing at a base or after time for long syntheses, and building step-by-step logs in real time. It then walks through Map mode, showing how columns can be added or removed at specific rep boundaries and how pause after rep and pause after cycle work. The video also covers trial monitor graphs, including V2, where values below 50 to 60 indicate reliable monitoring and higher V2 suggests unreliable first coupling.