video1.0<iframe src="https://www.loom.com/embed/9e6f375933f64987b2870bf4a71b7602" frameborder="0" width="1728" height="1296" webkitallowfullscreen mozallowfullscreen allowfullscreen></iframe>12961728Loomhttps://www.loom.com12961728https://cdn.loom.com/sessions/thumbnails/9e6f375933f64987b2870bf4a71b7602-50312e2b70802686.gif309.85This Loom explains how to configure port assignments by plumbing reagents and amidites to specific positions on the instrument manifold. It describes how the manifold view is split in the software layout and must match the physical delivery manifold, with the left side and right side representing different rows. The video notes that you cannot move columns to different manifold positions, and duplicate port assignments are not allowed, even though any reagent or amidite can be assigned to any non-duplicate position. It also covers the “Use Mixing Chamber” option, which is hard coded to one position and must be disengaged to free that place for another input.