video1.0<iframe src="https://www.loom.com/embed/df0dd1df38d74adca3c165caacb1c1b0" frameborder="0" width="1728" height="1296" webkitallowfullscreen mozallowfullscreen allowfullscreen></iframe>12961728Loomhttps://www.loom.com12961728https://cdn.loom.com/sessions/thumbnails/df0dd1df38d74adca3c165caacb1c1b0-2ac9918de5d3c73f.gif249.457This Loom explains how to use the flow rate screen to calculate consumption for synthesis runs and diagnose hardware issues across column positions. It captures samples for specified durations (for example, activator flowing for 3.3 seconds into waste tubing) and records the measured volume to compute total consumption based on protocol timing. For diagnostics, all positions are filled with ACN and flow should be relatively even within tolerance of plus or minus 5 percent, with expected variation due to bottle or vial shape and size. If one column shows about 20 percent less flow than others, it may indicate an obstruction, and the manifold should be purged between samples to prevent primed line residue from affecting results.