video1.0<iframe src="https://www.loom.com/embed/fb0d12fe8e354d9b9655ba07c6b33b98" frameborder="0" width="1728" height="1296" webkitallowfullscreen mozallowfullscreen allowfullscreen></iframe>12961728Loomhttps://www.loom.com12961728https://cdn.loom.com/sessions/thumbnails/fb0d12fe8e354d9b9655ba07c6b33b98-346b6f576ef0033f.gif164.104This Loom explains priming the manifold with fluids to eliminate dead space before synthesis begins. It covers auto-prime, which cycles through each position individually with the waste pathway engaged, using a programmable prime time of 350 milliseconds per position by default. The speaker recommends pulse prime when initially adding new bottles, watching the tube until fluid reaches the manifold, and notes hold prime as an alternative that actuates the valve while clicked. It also describes a mid-synthesis priming feature that primes for the same defined duration (350 ms here) before coupling, with optional adjustment for more viscous solutions or zero if the protocol already accounts for timing.